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Pierce,AminoLink Reductant

貨號:44892,名稱: AminoLink Reductant ,品牌:PIERCE,規格:2 X 1 G
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產品介紹

訂貨:,,

 

AminoLink Reductant, 2 x 1 g 介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

AminoLink Reductant, 2 x 1 g 現貨供應

 

PIERCE

44892

AminoLink Reductant, 2 x 1 g.

2 X 1 G

PIERCE

44894

AminoLink Plus Immobilization Kit

1 KIT

PIERCE

44897

IgM Purification Kit

EA

 

AminoLink Reductant, 2 x 1 g 品牌介紹

美國Pierce公司(www.pierce.com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

主要產品包括:

1)化學發光體和比色底物

 

2)分析試劑

 

3)熒光標記探針

 

4)酶聯免疫分析試劑盒等

 

AminoLink Reductant, 2 x 1 g 僅用于科研

 

Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

Proteins and other molecules with primary amines can be covalently attached (immobilized) to AminoLink Resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

 

Highlights:

  • AminoLink Coupling Resin – aldehyde-activated crosslinked, 4% beaded agarose
  • Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH2)
  • Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
  • Stable, permanent immobilization – Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
  • Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
  • Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions



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