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Pierce,EZ-Link (tm) Plus Activated Peroxidase Kit

貨號:31489,名稱: EZ-Link (tm) Plus Activated Peroxidase Kit ,品牌:PIERCE,規格:KIT
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產品介紹

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EZ-Link (tm) Plus Activated Peroxidase Kit 介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

EZ-Link (tm) Plus Activated Peroxidase Kit 現貨供應

 

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EZ-Link (tm) Plus Activated Peroxidase Kit 品牌介紹

美國Pierce公司(www.pierce.com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

主要產品包括:

1)化學發光體和比色底物

 

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3)熒光標記探針

 

4)酶聯免疫分析試劑盒等

 

EZ-Link (tm) Plus Activated Peroxidase Kit 僅用于科研

 

Pierce Plus Activated Peroxidase for making antibody-HRP conjugates.

Thermo Scientific Pierce Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins!

Sugar groups on native HRP have been oxidized with periodate to produce amine-reactive aldehydes. The activated peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and conjugate inactivation.

This high-quality prepared enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.

Highlights:

  • Reacts with readily accessible primary amines to form covalent secondary amine bonds with antibodies or other proteins
  • Provides more efficient and stable crosslinking than glutaraldehyde methods, resulting in conjugates having higher activity and overall performance in most applications
  • Can be stored for at least 12 months at -20°C
  • Enzyme activity is 120 to 200 units/mg



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