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PIERCE,Pierce Glycoprotein Staining Kit

貨號:24562,名稱: Pierce Glycoprotein Staining Kit ,品牌:PIERCE,規格:KIT
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Pierce Glycoprotein Staining  Kit介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

Pierce Glycoprotein Staining  Kit現貨供應

 

PIERCE

24562

Pierce Glycoprotein Staining  Kit

KIT

PIERCE

24590

Gelcode Blue Stain Reagent,   500 Ml. (see also #24592-3.5L)

500 ML

PIERCE

24596

GelCode(tm) Blue Safe Protein Stain, 3.5 L.

3.5 LITER

 

Pierce Glycoprotein Staining  Kit品牌介紹

美國Pierce公司(www.pierce.com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

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1)化學發光體和比色底物

 

2)分析試劑

 

3)熒光標記探針

 

4)酶聯免疫分析試劑盒等

 

Pierce Glycoprotein Staining  Kit僅用于科研

 

The Thermo Scientific Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

  • Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
  • Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
  • Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
  • Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16µg horseradish peroxidase per band
  • Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).
  • Gels or membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes



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