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PIERCE,(SMCC) Succinimidyl 4-(N-Maleimidomethyl)Cy

貨號:22360,名稱:(SMCC) Succinimidyl 4-(N-Maleimidomethyl)Cyclohexane-1-Carbo,規格:50 MG.
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(SMCC) Succinimidyl 4-(N-Maleimidomethyl)Cyclohexane-1-Carbo介紹

 

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

(SMCC) Succinimidyl 4-(N-Maleimidomethyl)Cyclohexane-1-Carbo現貨供應

 

PIERCE

22330

1,6-BISMALEIMIDOHEXANE, BMH

50 MG

PIERCE

22360

(SMCC) Succinimidyl 4-(N-Maleimidomethyl)Cyclohexane-1-Carbo

50 MG

PIERCE

22362

LC-SMCC:  Succinimidyl-4-(N-Maleimidomethyl)Cyclohexane-1-Ca

50 MG

 

(SMCC) Succinimidyl 4-(N-Maleimidomethyl)Cyclohexane-1-Carbo品牌介紹

 

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(SMCC) Succinimidyl 4-(N-Maleimidomethyl)Cyclohexane-1-Carbo

僅用于科研

Thermo Scientific Pierce SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms).

Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N -hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of SMCC and Sulfo-SMCC and are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm.

SMCC Highlights

  • Amine reactive NHS ester crosslinks rapidly with primary amine-containing molecule
  • Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
  • High purity, crystalline SMCC can be used to create high-purity maleimide-activated derivatives
  • Cyclohexane bridge confers added stability to the maleimide group making SMCC an ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C。

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