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PIERCE,Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 p

貨號:21650,名稱:Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro,品牌:PIERCE,規格:50 MG
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產品介紹

訂貨:,,

 

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro介紹

 

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro現貨供應

 

PIERCE

21627

Streptavidin, R-Phycoerythrin Conjugated, 1 mL.

1 ML

PIERCE

21650

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro

50 MG

PIERCE

21655

DISUCCINIMIDYL SUBERATE, (DSS)50 MG

50 MG

 

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro品牌介紹

 

美國Pierce公司(www.pierce.com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

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1)化學發光體和比色底物

 

2)分析試劑

 

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4)酶聯免疫分析試劑盒等

 

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro

僅用于科研

 

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

 

Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

 

Highlights:

  • Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
  • Water soluble, amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products

     

     

  • Sulfo-LC-SPDP crosslinker is not membrane permeable, so crosslinking can be carried out on the cell surface

     

     



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