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PIERCE,DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpro

貨號:21578,名稱:DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate),品牌:PIERCE,規格:50 MG
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DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)現貨供應

 

PIERCE

21578

DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)

50 MG

PIERCE

21580

BS3, Bis(Sulfosuccinimidyl) Suberate

50 MG

PIERCE

21585

BS3, No-Weigh(tm) Format,     8 x 2 mg microtubes

8 X 2 MG

 

DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)品牌介紹

美國Pierce公司(www.pierce.com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

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1)化學發光體和比色底物

 

2)分析試劑

 

3)熒光標記探針

 

4)酶聯免疫分析試劑盒等

 

DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)僅用于科研

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

3,3´-Dithiobis(sulfosuccinimidylpropionate)  (DTSSP) contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker. BS3, the non-cleavable analog of DTSSP is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

DTSSP Highlights

  • Amine reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products
  • DTSSP crosslinker is not membrane permeable, so cell-surface crosslinking can be done


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