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PIERCE,DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL

品牌:PIERCE,貨號:20665,產品名稱:DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP),1GM,規格: 1GM
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DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)介紹

 

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)現貨供應

 

PIERCE

20665

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP),1GM

1 GM

PIERCE

20688

Dimethyl Sulfoxide, Sequanal Grade (DMSO)

950 ML

PIERCE

21004

IgG Elution Buffer, 1 Liter

1 LITER

 

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)品牌介紹

 

美國Pierce公司(www.pierce.com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

主要產品包括:

1)化學發光體和比色底物

 

2)分析試劑

 

3)熒光標記探針

 

4)酶聯免疫分析試劑盒等

 

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)

僅用于科研

 

Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

 

Dimethyl dithiobispropionimidate (DTBP) is a homobifunctional, cleavable and membrane permeable cross-linker. It contains an amine-reactive imidoester at each end of an 8-atom spacer arm. Imidoesters react with primary amines at pH 8-10 to form stable amidine bonds. The amidine is protonated and therefore, like the lysine it modified, has a positive charge at physiological pH. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for imidoester crosslinking reagents.

 

DTBP crosslinks can be easily reversed by the addition of 100-150 mM DTT at 37°C for 30 minutes. DMS, the non-cleavable analog of DTBP is available for applications that require a stable, non-cleavable bond. DTSSP, the NHS-ester analog of DTBP is also available for applications that require crosslinking at physiologic pH or greater stability of the crosslinked product at elevated pH. DTBP and DMS have essentially identical crosslinking activity toward primary amines.

 

DTBP Highlights

  • Amine-reactive imidoester reacts rapidly with any primary amine-containing molecule to form amidine bonds
  • DTBP crosslinker is water soluble for compatibility with aqueous solutions
  • DTBP crosslinking can be easily reversed with DTT, TCEP or other reducing agents
  • High purity DTBP can be used to create high-purity conjugates
  • High purity DTBP can be used to create high-purity conjugates

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