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PIERCE,Pierce Nickel Coated, High Binding Capacity

品牌:PIERCE,貨號:15142,品名:Pierce Nickel Coated, High Binding Capacity Plates (Clear, 8-well strip),規格:5 PLATES
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Pierce Nickel Coated, High Binding Capacity Plates (Clear, 8-well strip)介紹:

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 Thermo Scientific Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Highlights:

  • Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
  • Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
  • Better binding for sensitive assays compared to other commercially available nickel-activated plates
  • Detection limit: 1ng of polyhistidine fusion protein
  • Binding capacity: approx. 9pmol His-tagged protein (27kDa) per well
  • Activation level: 200µL

 Pierce Nickel Coated, High Binding Capacity Plates (Clear, 8-well strip)品牌介紹:

美國Pierce公司(www.pierce.com)是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

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Pierce Nickel Coated, High Binding Capacity Plates (Clear, 8-well strip)

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